Anti-RNF3 (247 aa isoform) antibody (ab3892)

Overview

  • Product name
    Anti-RNF3 (247 aa isoform) antibody
  • Description
    Goat polyclonal to RNF3 (247 aa isoform)
  • Specificity
    This antibody is expected to recognise the 247 aa isoform (NP_006306) only, not the 132 aa isoform (NP_694982).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: PLLLHYRPKMDLL, corresponding to C terminal amino acids 230-242 of Human RNF3 (247 aa isoform).

  • Positive control
    • Human Heart lysate.
  • General notes
    Principal Names – RNF3; DONG1; RNF3A; MGC40413; ring finger protein 3 Official Gene Symbol - RNF3 GenBank Accession Number – NP_006306 LocusLink ID - 10336 (human); 69587 (mouse).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Can be blocked with Human RNF3 (247 aa isoform) peptide (ab22965).

Target

  • Function
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686D20235 antibody
    • DONG1 antibody
    • FLJ36550 antibody
    • FLJ43813 antibody
    • MGC129615 antibody
    • MGC40413 antibody
    • PCGF3 antibody
    • PCGF3_HUMAN antibody
    • polycomb group ring finger 3 antibody
    • Polycomb group RING finger protein 3 antibody
    • Ring finger protein 3 antibody
    • RING finger protein 3A antibody
    • RNF3 antibody
    • RNF3A antibody
    see all

Images

  • ab3892 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

ab3892 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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