Overview

  • Product nameAnti-RNF31/HOIP antibody
    See all RNF31/HOIP primary antibodies
  • Description
    Rabbit polyclonal to RNF31/HOIP
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1022-1072 of Human RNF31/HOIP (NP_060469.4).

  • Positive control
    • HeLa whole cell lysate Jurkat whole cell lysate 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab125189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 120 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
    • Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
    • Sequence similaritiesContains 2 IBR-type zinc fingers.
      Contains 3 RanBP2-type zinc fingers.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 ubiquitin protein ligase RNF31 antibody
      • FLJ10111 antibody
      • FLJ23501 antibody
      • HOIL 1L interacting protein antibody
      • HOIL-1-interacting protein antibody
      • HOIL1L interacting protein antibody
      • HOIP antibody
      • RING finger protein 31 antibody
      • RNF31 antibody
      • RNF31_HUMAN antibody
      • ZIBRA antibody
      • Zinc in between ring finger ubiquitin associated domain antibody
      • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
      see all

    Anti-RNF31/HOIP antibody images

    • All lanes : Anti-RNF31/HOIP antibody (ab125189) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 120 kDa


      Exposure time : 3 minutes

    References for Anti-RNF31/HOIP antibody (ab125189)

    ab125189 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Sample Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
    Specification HEK293 and hTERT-RPE1
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 31 2014

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Specification Mouse embryonic fibroblasts
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"