Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 123 kDa.Can be blocked with Human RNF31/HOIP peptide (ab22938).

Target

  • FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
  • Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
  • Sequence similaritiesContains 2 IBR-type zinc fingers.
    Contains 3 RanBP2-type zinc fingers.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.
  • DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase RNF31 antibody
    • FLJ10111 antibody
    • FLJ23501 antibody
    • HOIL 1L interacting protein antibody
    • HOIL-1-interacting protein antibody
    • HOIL1L interacting protein antibody
    • HOIP antibody
    • RING finger protein 31 antibody
    • RNF31 antibody
    • RNF31_HUMAN antibody
    • ZIBRA antibody
    • Zinc in between ring finger ubiquitin associated domain antibody
    • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
    see all

Anti-RNF31/HOIP antibody images

  • Anti-RNF31/HOIP antibody (ab2919) at 2 µg/ml + HepG2 lysate (RIPA buffer, 30µg total protein per lane).

    Predicted band size : 123 kDa


    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-RNF31/HOIP antibody (ab2919)

This product has been referenced in:

See 1 Publication for this product

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