Overview

  • Product nameAnti-RNF31/HOIP antibody
    See all RNF31/HOIP primary antibodies
  • Description
    Rabbit polyclonal to RNF31/HOIP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to amino acids 1008-1057 (SLINAHSLDP ATLYEVEELE TATERYLHVR PQPLAGEDPP AYQARLLQKL) of human RNF31/HOIP (NP_060469).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionE3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
  • Tissue specificityExpressed in both normal and transformed breast epithelial cell lines.
  • Sequence similaritiesContains 2 IBR-type zinc fingers.
    Contains 3 RanBP2-type zinc fingers.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.
  • DomainThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase RNF31 antibody
    • FLJ10111 antibody
    • FLJ23501 antibody
    • HOIL 1L interacting protein antibody
    • HOIL-1-interacting protein antibody
    • HOIL1L interacting protein antibody
    • HOIP antibody
    • RING finger protein 31 antibody
    • RNF31 antibody
    • RNF31_HUMAN antibody
    • ZIBRA antibody
    • Zinc in between ring finger ubiquitin associated domain antibody
    • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
    see all

Anti-RNF31/HOIP antibody images

  • Anti-RNF31/HOIP antibody (ab85294) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 120 kDa
    Observed band size : 120 kDa

References for Anti-RNF31/HOIP antibody (ab85294)

This product has been referenced in:
  • Yang Y  et al. Essential role of the linear ubiquitin chain assembly complex in lymphoma revealed by rare germline polymorphisms. Cancer Discov 4:480-93 (2014). Read more (PubMed: 24491438) »
  • Blackwell K  et al. Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate and delayed I?B kinase activation. Mol Cell Biol 33:1901-15 (2013). Read more (PubMed: 23459942) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
Specification HEK293 and hTERT-RPE1
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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Submitted Mar 27 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

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Verified customer

Submitted Mar 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"