Overview

  • Product nameAnti-RNF34 antibody
    See all RNF34 primary antibodies
  • Description
    Rabbit polyclonal to RNF34
  • SpecificityExpect reactivity with isoform 1 and 2 of RNF34.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • RNF34 transfected lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the given buffer.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.

Target

  • FunctionE3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
  • Tissue specificityUbiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • DomainThe RING-type zinc finger is required for the ubiquitination of target proteins.
    The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
  • Post-translational
    modifications
    Autoubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • Cellular localizationCell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARP 1 antibody
    • CARP-1 antibody
    • CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspases-8 and -10-associated RING finger protein 1 antibody
    • E3 ubiquitin-protein ligase RNF34 antibody
    • FYVE-RING finger protein Momo antibody
    • hRFI antibody
    • Human RING finger homologous to inhibitor of apoptosis protein antibody
    • RFI antibody
    • RIF antibody
    • RIFF antibody
    • RING finger protein 34 antibody
    • RING finger protein RIFF antibody
    • RNF34 antibody
    • RNF34_HUMAN antibody
    see all

Anti-RNF34 antibody images

  • All lanes : Anti-RNF34 antibody (ab47255) at 1/1000 dilution

    Lane 1 : Empty vector 293T cell lysate
    Lane 2 : RNF34 transfected 293T cell lysate
    Lane 3 : RFFL transfected 293T cell lysate

    Lysates/proteins at 20 µg/ml per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-RNF34 antibody (ab47255)

ab47255 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab47255.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"