Anti-RNF4 antibody (ab230438)
Key features and details
- Rabbit polyclonal to RNF4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF4 antibody -
Description
Rabbit polyclonal to RNF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RNF4 aa 95-123 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P78317 -
Positive control
- WB: LNCaP cell lysate. IHC-P: Human colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation. -
Tissue specificity
Widely expressed at low levels in many tissues; highly expressed in testis. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates. -
Post-translational
modificationsSumoylated; conjugated by one or two SUMO1 moieties.
Autoubiquitinated. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > PML body. Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6047 Human
- Omim: 602850 Human
- SwissProt: P78317 Human
- Unigene: 443116 Human
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Alternative names
- E3 ubiquitin ligase RNF4 antibody
- E3 ubiquitin protein ligase RNF4 antibody
- Protein SNURF antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab230438 has not yet been referenced specifically in any publications.