The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa). Abcam recommends using 5% milk as the blocking agent.
FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.
Tissue specificityUbiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family. Contains 1 RING-type zinc finger.
Immunocytochemistry/ Immunofluorescence - RNF40 antibody (ab26082)Image courtesy of an abreview submitted by Kirk McManus, University of Manitoba/MICB, Canada
ab26082 (1/200) staining RNF40 in Human HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized using 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.
Long L et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem289:8916-30 (2014).
Read more (PubMed: 24526689) »
Lilley CE et al. A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses. EMBO J29:943-55 (2010).
Read more (PubMed: 20075863) »