Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab26082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa). Abcam recommends using 5% milk as the blocking agent.
ICC/IF 1/200.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.
  • Tissue specificityUbiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 kDa retinoblastoma associated protein antibody
    • 95 kDa retinoblastoma protein binding protein antibody
    • 95 kDa retinoblastoma-associated protein antibody
    • BRE 1B antibody
    • BRE1 B antibody
    • BRE1 E3 ubiquitin ligase homolog B antibody
    • BRE1-B antibody
    • BRE1B antibody
    • BRE1B_HUMAN antibody
    • DKFZp686K191 antibody
    • E3 ubiquitin protein ligase BRE1B antibody
    • E3 ubiquitin-protein ligase BRE1B antibody
    • KIAA0661 antibody
    • MGC13051 antibody
    • Rb associated protein antibody
    • RBP 95 antibody
    • RBP95 antibody
    • RING finger protein 40 antibody
    • Ring finger protein 40 E3 ubiquitin protein ligase antibody
    • RNF 40 antibody
    • Rnf40 antibody
    • STARING antibody
    see all

Anti-RNF40 antibody images

  • Anti-RNF40 antibody (ab26082) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 35 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 114 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab26082 (1/200) staining RNF40 in Human HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized using 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.

    See Abreview

References for Anti-RNF40 antibody (ab26082)

This product has been referenced in:
  • Long L  et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem 289:8916-30 (2014). WB . Read more (PubMed: 24526689) »
  • Lilley CE  et al. A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses. EMBO J 29:943-55 (2010). WB ; Human . Read more (PubMed: 20075863) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (H1299)
Loading amount 25 µg
Specification H1299
Treatment Control or RNF40 siRNA for 72 h.
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Steven Johnsen

Verified customer

Submitted Aug 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 29 2010

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (U2OS Cells stably transfected w/ estrogen receptor)
Specification U2OS Cells stably transfected w/ estrogen receptor
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Real-time PCR
Positive control None
Negative control Tested 2 sites - one at the promoter region and one within the transcribed region
Username

Abcam user community

Verified customer

Submitted Jan 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa Cells)
Specification HeLa Cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MDA-MB-231 Cells)
Total protein in input 2e+006 cells
Specification MDA-MB-231 Cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Jan 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"