Overview

  • Product nameAnti-RNF40 antibody
    See all RNF40 primary antibodies
  • Description
    Rabbit polyclonal to RNF40
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: KLEEKERALQ GSLGGVEKEL TLRSQALELN KRKAVEAAQL AEDLKVQLEH , corresponding to C terminal amino acids 829-878 of Human RNF40

  • Positive control
    • 293T cell lysate. Human muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 114 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.
  • Tissue specificityUbiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 kDa retinoblastoma associated protein antibody
    • 95 kDa retinoblastoma protein binding protein antibody
    • 95 kDa retinoblastoma-associated protein antibody
    • BRE 1B antibody
    • BRE1 B antibody
    • BRE1 E3 ubiquitin ligase homolog B antibody
    • BRE1-B antibody
    • BRE1B antibody
    • BRE1B_HUMAN antibody
    • DKFZp686K191 antibody
    • E3 ubiquitin protein ligase BRE1B antibody
    • E3 ubiquitin-protein ligase BRE1B antibody
    • KIAA0661 antibody
    • MGC13051 antibody
    • Rb associated protein antibody
    • RBP 95 antibody
    • RBP95 antibody
    • RING finger protein 40 antibody
    • Ring finger protein 40 E3 ubiquitin protein ligase antibody
    • RNF 40 antibody
    • Rnf40 antibody
    • STARING antibody
    see all

Anti-RNF40 antibody images

  • Anti-RNF40 antibody (ab62528) at 1.25 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 114 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • ab62528, at 4-8ug/ml, staining RNF40 in human muscle tissue by immunohistochemistry using paraffin-embedded tissue. The arrows indicate positively stained skeletal muscle cells.

References for Anti-RNF40 antibody (ab62528)

This product has been referenced in:
  • Lee YJ  et al. E3 ubiquitin-protein ligases in rat kidney collecting duct: response to vasopressin stimulation and withdrawal. Am J Physiol Renal Physiol 301:F883-96 (2011). WB ; Rat . Read more (PubMed: 21734099) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"