Overview

  • Product nameAnti-RNF40 antibody
    See all RNF40 primary antibodies
  • Description
    Mouse monoclonal to RNF40
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DETVEALLRC HESQGELSSA PEAPGTQEGP TCDGTPLPEP GTSELRDPLL MQLRPPLSEP ALAFVVALGA SSSEEVELEL QGRMEFSKAA VSRVVEASD, corresponding to amino acids 102-200 of Human RNF40 (NP_055586)

  • Positive control
    • RNF40 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 1ng/ml as a capture antibody when used against the immunogen.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.
  • Tissue specificityUbiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 kDa retinoblastoma associated protein antibody
    • 95 kDa retinoblastoma protein binding protein antibody
    • 95 kDa retinoblastoma-associated protein antibody
    • BRE 1B antibody
    • BRE1 B antibody
    • BRE1 E3 ubiquitin ligase homolog B antibody
    • BRE1-B antibody
    • BRE1B antibody
    • BRE1B_HUMAN antibody
    • DKFZp686K191 antibody
    • E3 ubiquitin protein ligase BRE1B antibody
    • E3 ubiquitin-protein ligase BRE1B antibody
    • KIAA0661 antibody
    • MGC13051 antibody
    • Rb associated protein antibody
    • RBP 95 antibody
    • RBP95 antibody
    • RING finger protein 40 antibody
    • Ring finger protein 40 E3 ubiquitin protein ligase antibody
    • RNF 40 antibody
    • Rnf40 antibody
    • STARING antibody
    see all

Anti-RNF40 antibody images

  • Anti-RNF40 antibody (ab77211) at 5 µg/ml

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RNF40 antibody (ab77211) at 5 µg/ml

    Lane 1 : RNF40 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 114 kDa

References for Anti-RNF40 antibody (ab77211)

ab77211 has not yet been referenced specifically in any publications.

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