The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
Use at an assay dependent dilution. Detection limit is approximately 1ng/ml as a capture antibody when used against the immunogen.
FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.
Tissue specificityUbiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family. Contains 1 RING-type zinc finger.