• Product nameAnti-RNF43 antibody
    See all RNF43 primary antibodies
  • Description
    Rabbit polyclonal to RNF43
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide designed within residues: DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH, corresponding to internal sequence amino acids 504 - 553 of Human RNF43 (NP_060233)

  • Positive control
    • MCF7 cell lysate.



Our Abpromise guarantee covers the use of ab84125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.


  • RelevanceFunction: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Tissue specificity: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ZNRF3 family. Contains 1 RING-type zinc finger. PTM: Autoubiquitinated.
  • Cellular localizationEndoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note: may be secreted.
  • Database links
  • Alternative names
    • 4732452J19Rik antibody
    • DKFZp781H02126 antibody
    • DKFZp781H0392 antibody
    • E3 ubiquitin-protein ligase RNF43 antibody
    • FLJ20315 antibody
    • FLJ77466 antibody
    • FLJ99338 antibody
    • MGC125630 antibody
    • RGD1305204 antibody
    • RING finger protein 43 antibody
    • RNF124 antibody
    • Rnf43 antibody
    • RNF43_HUMAN antibody
    • RP23-449P23.1 antibody
    • SSPCS antibody
    • URCC antibody
    see all

Anti-RNF43 antibody images

  • Anti-RNF43 antibody (ab84125) at 1 µg/ml + MCF7 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab84125 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RNF43 antibody (ab84125)

ab84125 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Gel Running Conditions Reduced Denaturing
Loading amount 5 µg
Specification mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jul 14 2015