Overview

  • Product nameAnti-RNF6 antibody
    See all RNF6 primary antibodies
  • Description
    Mouse polyclonal to RNF6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-685 of Human RNF6

  • Positive control
    • RNF6 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.
  • Tissue specificityWeakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in RNF6 may be a cause of esophageal cancer (ESCR) [MIM:133239]. A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.
  • Sequence similaritiesBelongs to the RNF12 family.
    Contains 1 RING-type zinc finger.
  • Developmental stageExpressed in embryo and adult testis.
  • Cellular localizationNucleus. Cytoplasm. Cell projection > axon. Nucleus > PML body. Localizes to the PML nuclear bodies in Sertoli cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RNF6 antibody
    • Ring finger protein (C3H2C3 type) 6 antibody
    • Ring finger protein 6 antibody
    • RING H2 protein antibody
    • RING H2 protein RNF6 antibody
    • RNF 6 antibody
    • RNF6 antibody
    • RNF6_HUMAN antibody
    • SPG2 antibody
    see all

Anti-RNF6 antibody images

  • All lanes : Anti-RNF6 antibody (ab76773) at 1/500 dilution

    Lane 1 : RNF6 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RNF6 antibody (ab76773)

ab76773 has not yet been referenced specifically in any publications.

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