Overview

  • Product nameAnti-RNF7 antibody
    See all RNF7 primary antibodies
  • Description
    Rabbit polyclonal to RNF7
  • SpecificitySufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.
  • Tested applicationsSuitable for: ELISA, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CQQDWVVQRIGK

    conjugated to KLH, corresponding to C terminal amino acids 102-113 of Human RNF7

Properties

Applications

Our Abpromise guarantee covers the use of ab34873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
Flow Cyt Use at an assay dependent concentration. PubMed: 24786833ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 12.6 kDa (predicted molecular weight: 13 kDa).

Target

  • FunctionProbable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
  • Tissue specificityExpressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Post-translational
    modifications
    Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CKBBP 1 antibody
    • CKBBP1 antibody
    • CKII beta binding protein 1 antibody
    • CKII beta-binding protein 1 antibody
    • Rbx 2 antibody
    • Rbx2 antibody
    • RBX2_HUMAN antibody
    • Regulator of cullins 2 antibody
    • RING box protein 2 antibody
    • RING finger protein 7 antibody
    • RING-box protein 2 antibody
    • RNF 7 antibody
    • RNF7 antibody
    • ROC 2 antibody
    • ROC2 antibody
    • SAG antibody
    • Sensitive to apoptosis gene antibody
    • Sensitive to apoptosis gene protein antibody
    • Zinc RING finger protein SAG antibody
    see all

References for Anti-RNF7 antibody (ab34873)

This product has been referenced in:
  • Chang SC & Ding JL Ubiquitination by SAG regulates macrophage survival/death and immune response during infection. Cell Death Differ N/A:N/A (2014). Flow Cyt . Read more (PubMed: 24786833) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"