Anti-RNF7 antibody (ab99284)

Overview

  • Product name
  • Description
    Goat polyclonal to RNF7
  • Specificity
    ab99284 is expected to recognize isoform 1 (NP_055060.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    DACLRCQAENKQE

    , corresponding to internal sequence amino acids 59-71 of Human RNF7 (NP_055060.1).

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 13 kDa). An approximately 11 kDa band was observed in Human Heart lysates. An additional band of 28 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Target

  • Function
    Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
  • Tissue specificity
    Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Post-translational
    modifications
    Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CKBBP 1 antibody
    • CKBBP1 antibody
    • CKII beta binding protein 1 antibody
    • CKII beta-binding protein 1 antibody
    • Rbx 2 antibody
    • Rbx2 antibody
    • RBX2_HUMAN antibody
    • Regulator of cullins 2 antibody
    • RING box protein 2 antibody
    • RING finger protein 7 antibody
    • RING-box protein 2 antibody
    • RNF 7 antibody
    • RNF7 antibody
    • ROC 2 antibody
    • ROC2 antibody
    • SAG antibody
    • Sensitive to apoptosis gene antibody
    • Sensitive to apoptosis gene protein antibody
    • Zinc RING finger protein SAG antibody
    see all

Anti-RNF7 antibody images

  • Anti-RNF7 antibody (ab99284) at 1 µg/ml + Human heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 13 kDa

References for Anti-RNF7 antibody (ab99284)

ab99284 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

This antibody has not been tested with Drosophila samples so I am sorry we may not be able to confirm its specificity. I however advise you to run a human cell line lysates as positive control - this will help to figu...

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Sorry! the species is untested. So a positive control from human lysates will be a good idea to try.

Thank you for submitting an Abreview of ab99284. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on ...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Embryos)
Loading amount
50 µg
Specification
Embryos
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

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Verified customer

Submitted Nov 23 2012

Thank you for your email.


As per our email correspondence, you had previously requested a refund which had been issued and was processed on xxx with Credit Note reference xxx. At this point, I do not think that further troubleshooting w...

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I am very pleased to hear you would like to test ab99284 in drosophila. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for drosophila and include this code in the “Addi...

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