Recombinant
RabMAb

Anti-RNF7 antibody [EPR12001] (ab181986)

Overview

  • Product name
    Anti-RNF7 antibody [EPR12001]
    See all RNF7 primary antibodies
  • Description
    Rabbit monoclonal [EPR12001] to RNF7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RNF7 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UBF6

  • Positive control
    • HepG2, Mouse kidney or Mouse heart lysate, Human transitional cell carcinoma of bladder or Mouse liver tissue, HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.

WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
ICC/IF 1/100.

Target

  • Function
    Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
  • Tissue specificity
    Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Post-translational
    modifications
    Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CKBBP 1 antibody
    • CKBBP1 antibody
    • CKII beta binding protein 1 antibody
    • CKII beta-binding protein 1 antibody
    • Rbx 2 antibody
    • Rbx2 antibody
    • RBX2_HUMAN antibody
    • Regulator of cullins 2 antibody
    • RING box protein 2 antibody
    • RING finger protein 7 antibody
    • RING-box protein 2 antibody
    • RNF 7 antibody
    • RNF7 antibody
    • ROC 2 antibody
    • ROC2 antibody
    • SAG antibody
    • Sensitive to apoptosis gene antibody
    • Sensitive to apoptosis gene protein antibody
    • Zinc RING finger protein SAG antibody
    see all

Images

  • Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

  • Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder  tissue labeling RNF7 with
    ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

References

This product has been referenced in:
  • Xiao Y  et al. RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway. Sci Rep 7:43683 (2017). WB . Read more (PubMed: 28252001) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 04 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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