Overview

  • Product nameAnti-RNF8 antibody
    See all RNF8 primary antibodies
  • Description
    Rabbit polyclonal to RNF8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid peptide near the C terminus of Human RNF8 (NP_003949).

  • Positive control
    • WB: Human lung tissue lysate ICC/IF: Rat lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionE3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
  • Tissue specificityUbiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RNF8 family.
    Contains 1 FHA domain.
    Contains 1 RING-type zinc finger.
  • Developmental stageLow levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.
  • DomainThe FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.
  • Post-translational
    modifications
    Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
  • Cellular localizationNucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.
  • Information by UniProt
  • Database links
  • Alternative names
    • C3HC4 type zinc finger protein antibody
    • E3 ubiquitin protein ligase RNF8 antibody
    • E3 ubiquitin-protein ligase RNF8 antibody
    • FLJ12013 antibody
    • KIAA0646 antibody
    • Ring finger protein (C3HC4 type) 8 antibody
    • RING finger protein 8 antibody
    • Ring finger protein 8, E3 ubiquitin protein ligase antibody
    • RNF 8 antibody
    • RNF8 antibody
    • RNF8_HUMAN antibody
    • UBC13/UEV interacting ring finger protein antibody
    see all

Anti-RNF8 antibody images

  • Lane 1 : Anti-RNF8 antibody (ab105362) at 1 µg/ml
    Lane 2 : Anti-RNF8 antibody (ab105362) at 2 µg/ml

    Lane 1 : Human lung tissue lysate
    Lane 2 : Human lung tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 56 kDa
  • Immunofluorescent analysis of RNF8 in Rat lung tissue using ab105362 at 20 µg/ml.
  • Immunofluorescence of RNF8 in Rat Lung cells using ab105362 at 20 ug/ml.

References for Anti-RNF8 antibody (ab105362)

ab105362 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (EC9706)
Permeabilization Yes - 0.1% Triton X-100
Specification EC9706
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Formaldehyde
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Submitted Jan 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (esophageal tumor cells&293T)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%SDS)
Loading amount 80 µg
Specification esophageal tumor cells&293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Dec 31 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Mouse Cell lysate - whole cell (MEF)
Specification MEF
Treatment with/without IR 20Gy 1hr
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"