Overview

  • Product nameAnti-RNF86 antibody [4E5]
    See all RNF86 primary antibodies
  • Description
    Mouse monoclonal [4E5] to RNF86
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-100 and 645-744 of Human RNF86 produced in HEK293T cells (NP_056086).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY RNF86 cDNA; PC12 cell extracts; HeLa cells
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab123899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 82 kDa.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay contribute to the alteration of neural cellular mechanisms.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • DomainThe interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase TRIM2 antibody
    • KIAA0517 antibody
    • RING finger protein 86 antibody
    • RNF86 antibody
    • Trim2 antibody
    • TRIM2_HUMAN antibody
    • Tripartite motif containing 2 antibody
    • Tripartite motif protein 2 antibody
    • Tripartite motif protein TRIM2 antibody
    • Tripartite motif-containing 2 antibody
    • Tripartite motif-containing protein 2 antibody
    see all

Anti-RNF86 antibody [4E5] images

  • All lanes : Anti-RNF86 antibody [4E5] (ab123899) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfec ted with pCMV6-ENTRY RNF86 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 82 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203947)
  • All lanes : Anti-RNF86 antibody [4E5] (ab123899) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 82 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203947)
  • Flow cytometric analysis of HeLa cells, using ab123899 at 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).

References for Anti-RNF86 antibody [4E5] (ab123899)

ab123899 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123899.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"