Overview

  • Product name
  • Description
    Goat polyclonal to RNF86
  • Tested applications
    Suitable for: IP, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    DSGNHCFKVYRYLQ

    , corresponding to C terminal amino acids 731-744 of Human RNF86.

Properties

Applications

Our Abpromise guarantee covers the use of ab3942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml.
ELISA Use at an assay dependent dilution. Peptide ELISA (antibody detection limit dilution 1:16,000).

Target

  • Function
    May contribute to the alteration of neural cellular mechanisms.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Domain
    The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase TRIM2 antibody
    • KIAA0517 antibody
    • RING finger protein 86 antibody
    • RNF86 antibody
    • Trim2 antibody
    • TRIM2_HUMAN antibody
    • Tripartite motif containing 2 antibody
    • Tripartite motif protein 2 antibody
    • Tripartite motif protein TRIM2 antibody
    • Tripartite motif-containing 2 antibody
    • Tripartite motif-containing protein 2 antibody
    see all

Images

  • Anti-RNF86 antibody (ab3942) at 0.5 µg/ml + Tissue lysates prepared from Rat brain at 35 µg

    Observed band size : 80,85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab3942 at 6.3 µg/ml staining RNF86 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab3942 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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