Overview

  • Product name
  • Description
    Goat polyclonal to RNF87
  • Specificity
    This antibody is expected to recognise both human isoforms.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: ASLVIPPVTDRK, corresponding to C terminal amino acids 489-500 of Human RNF87.

  • General notes
    GenBank Accession Number – NP_148977; NP_149082.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: 1/32000.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55-58 kDa (predicted molecular weight: 55-60 kDa according to NP_148977 and NP_149082).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 B30.2/SPRY domain.
      Contains 1 RING-type zinc finger.
    • Information by UniProt
    • Database links
    • Alternative names
      • RING finger protein 87 antibody
      • RNF 87 antibody
      • TRIM4 antibody
      • TRIM4_HUMAN antibody
      • Tripartite motif containing 4 antibody
      • Tripartite motif protein 4 antibody
      • Tripartite motif protein TRIM4 antibody
      • Tripartite motif-containing protein 4 antibody
      see all

    Images



    • Predicted band size : 55-60 kDa


      ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

    References

    This product has been referenced in:
    • Feyeux M  et al. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Hum Mol Genet 21:3883-95 (2012). Read more (PubMed: 22678061) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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