Overview

  • Product nameAnti-RNF93 antibody
    See all RNF93 primary antibodies
  • Description
    Rabbit polyclonal to RNF93
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Chimpanzee
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: EDVKCQEDQK LQVLLTQIES KKHQVETAFE RLQQELEQQR CLLLARLREL, corresponding to N terminal amino acids 145-194 of Human RNF93

  • Positive control
    • HepG2 cell lysate or human kidney or intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab24335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Anti-RNF93 antibody images

  • Anti-RNF93 antibody (ab24335) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling RNF93 with ab24335 at 4µg/ml. Epithelial cells of the renal tubule (indicated with arrows) are positively stained.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RNF93 with ab24335 at 4µg/ml. Epithelial cells of the intestinal vilus (indicated with arrows) are positively stained.

References for Anti-RNF93 antibody (ab24335)

ab24335 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"