Overview

  • Product nameAnti-RNF98 antibody
    See all RNF98 primary antibodies
  • Description
    Rabbit polyclonal to RNF98
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Marmoset (common)
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 166-215 (ESTKSCMDCS ASYCNECFKI YHPWGTVKAQ HEYVGPTTNF RPKILMCPEH) of Rat RNF98 (NP_001099617.1).

  • Positive control
    • Rat lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionE3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
  • Tissue specificityHighly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosome RBCC protein antibody
    • E3 ubiquitin protein ligase TRIM36 antibody
    • E3 ubiquitin-protein ligase Trim36 antibody
    • Haprin antibody
    • RBCC728 antibody
    • RING finger protein 98 antibody
    • TRI36_HUMAN antibody
    • TRIM 36 antibody
    • Trim36 antibody
    • Tripartite motif containing 36 antibody
    • Tripartite motif containing protein 36 antibody
    • Tripartite motif protein 36 antibody
    • Tripartite motif-containing protein 36 antibody
    • Zinc binding protein Rbcc728 antibody
    • Zinc-binding protein Rbcc728 antibody
    see all

Anti-RNF98 antibody images

  • IHC-P image of RNF98 staining with ab116212 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab116212 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated Goat anti-Rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

  • Anti-RNF98 antibody (ab116212) at 1 µg/ml + Rat lung lysate at 10 µg

    Predicted band size : 83 kDa
    Gel concentration 12%

References for Anti-RNF98 antibody (ab116212)

ab116212 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult Testis)
Specification Adult Testis
Permeabilization No
Fixative Formaldehyde
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Submitted Oct 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"