Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF98
  • Host species
    Rabbit
  • Specificity
    ab171732 recognizes Isoform 1of Human RNF98.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RNF98 aa 309-339 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NQ86

  • Positive control
    • Human pancreas tissue, 293 cell line lysate and mouse bladder tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab171732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 83 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
  • Tissue specificity
    Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosome RBCC protein antibody
    • E3 ubiquitin protein ligase TRIM36 antibody
    • E3 ubiquitin-protein ligase Trim36 antibody
    • Haprin antibody
    • RBCC728 antibody
    • RING finger protein 98 antibody
    • TRI36_HUMAN antibody
    • TRIM 36 antibody
    • Trim36 antibody
    • Tripartite motif containing 36 antibody
    • Tripartite motif containing protein 36 antibody
    • Tripartite motif protein 36 antibody
    • Tripartite motif-containing protein 36 antibody
    • Zinc binding protein Rbcc728 antibody
    • Zinc-binding protein Rbcc728 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling RNF98 using ab171732 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-RNF98 antibody (ab171732) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Predicted band size: 83 kDa

  • Anti-RNF98 antibody (ab171732) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size: 83 kDa

References

ab171732 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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