Recombinant
RabMAb

Anti-robld3 antibody [EPR14378] (ab183514)

Overview

  • Product name
    Anti-robld3 antibody [EPR14378]
    See all robld3 primary antibodies
  • Description
    Rabbit monoclonal [EPR14378] to robld3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human robld3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y2Q5

  • Positive control
    • HepG2, Raji and LnCaP cell lysates, Human thyroid tissue and Human gastric carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 14 kDa.
IHC-P 1/50.

Target

  • Function
    As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
  • Involvement in disease
    Defects in LAMTOR2 are the cause of immunodeficiency due to defect in MAPBP-interacting protein (ID-MAPBPIP) [MIM:610798]. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency.
  • Sequence similarities
    Belongs to the GAMAD family.
  • Cellular localization
    Late endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ENDAP antibody
    • Endosomal adaptor protein p14 antibody
    • HSPC003 antibody
    • LAMTOR2 antibody
    • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2 antibody
    • Late endosomal/lysosomal Mp1 interacting protein antibody
    • Late endosomal/lysosomal Mp1-interacting protein antibody
    • LTOR2_HUMAN antibody
    • MAPBPIP antibody
    • MAPKSP1 adaptor protein antibody
    • MAPKSP1AP antibody
    • Mitogen activated protein binding protein interacting protein antibody
    • Mitogen-activated protein-binding protein-interacting protein antibody
    • p14 antibody
    • Ragulator complex protein LAMTOR2 antibody
    • Ragulator2 antibody
    • Roadblock domain containing 3 antibody
    • Roadblock domain containing protein 3 antibody
    • Roadblock domain-containing protein 3 antibody
    • ROBLD 3 antibody
    • RP11 336K24.9 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling robld3 using ab183514 at a 1/50 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human thyroid tissue labeling robld3 using ab183514 at a 1/50 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

  • All lanes : Anti-robld3 antibody [EPR14378] (ab183514) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 14 kDa

References

ab183514 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab183514.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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