Overview

  • Product name
  • Description
    Goat polyclonal to Robo1
  • Specificity
    ab11726 does not cross react with Robo2 or Robo3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide:

    QKARPAKKQKHQ

    by a Cysteine residue linker, corresponding to internal sequence amino acids 1417-1428 of Mouse Robo1 (NP_062286.2)

  • Positive control
    • Mouse Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab111726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 181 kDa).

Target

  • Function
    Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
  • Tissue specificity
    Widely expressed, with exception of kidney.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Ubiquitinated. May be deubiquitinated by USP33.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deleted in U twenty twenty antibody
    • DUTT 1 antibody
    • DUTT1 antibody
    • FLJ21882 antibody
    • H Robo 1 antibody
    • H-Robo-1 antibody
    • hRobo 1 antibody
    • Robo 1 antibody
    • Robo1 antibody
    • ROBO1_HUMAN antibody
    • Roundabout 1 antibody
    • Roundabout axon guidance receptor homolog 1 antibody
    • Roundabout homolog 1 antibody
    • Roundabout homolog1 precurser antibody
    • Roundabout1 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo1 antibody images

  • Anti-Robo1 antibody (ab111726) at 0.05 µg/ml + Mouse Fetal brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 181 kDa
  • All lanes : Anti-Robo1 antibody (ab111726) at 500 µg/ml

    Lane 1 : Blank
    Lane 2 : HEK293 cells expressing Robo1
    Lane 3 : HEK293 cells expressing Robo2
    Lane 4 : HEK293 cells expressing Robo3

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-goat IgG conjugated to HRP at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 181 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an abreview submitted by Alain Chedotal, Dépt. of Dev, Institut Vision, France

    See Abreview

References for Anti-Robo1 antibody (ab111726)

ab111726 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Oct 15 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
20 µg
Specification
HEK293 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Oct 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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