Overview

  • Product nameAnti-Robo1 antibody
    See all Robo1 primary antibodies
  • Description
    Mouse monoclonal to Robo1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: DGVLVSTQDS RIKQLENGVL QIRYAKLGDT GRYTCIASTP SGEATWSAYI EVQEFGVPVQ PPRPTDPNLI PSAPSKPEVT DVSRNTVTLS WQPNLNSGA, corresponding to amino acids 491-590 of Human Robo1

  • Positive control
    • mouse brain: embryonic day 16, three-month old brain, five-week old brain

Properties

Applications

Our Abpromise guarantee covers the use of ab58297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 180 kDa).

Target

  • FunctionReceptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
  • Tissue specificityWidely expressed, with exception of kidney.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Ubiquitinated. May be deubiquitinated by USP33.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deleted in U twenty twenty antibody
    • DUTT 1 antibody
    • DUTT1 antibody
    • FLJ21882 antibody
    • H Robo 1 antibody
    • H-Robo-1 antibody
    • hRobo 1 antibody
    • Robo 1 antibody
    • Robo1 antibody
    • ROBO1_HUMAN antibody
    • Roundabout 1 antibody
    • Roundabout axon guidance receptor homolog 1 antibody
    • Roundabout homolog 1 antibody
    • Roundabout homolog1 precurser antibody
    • Roundabout1 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo1 antibody images



  • Predicted band size : 180 kDa
    Western blot against tagged recombinant protein immunogen using ab58297 Robo1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • All lanes : Anti-Robo1 antibody (ab58297) at 1/500 dilution

    Lane 1 : mouse brain (embryonic day 12)
    Lane 2 : mouse brain (embryonic day 16)
    Lane 3 : mouse brain (three-month old)
    Lane 4 : mouse brain (5 week old)

    Secondary
    DAKO Cytomation, Polyclonal rabbit anti goat P0449 Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 180 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100& 75 kDa (possible non-specific binding),50& 37 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

References for Anti-Robo1 antibody (ab58297)

ab58297 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Gel Running Conditions Reduced Denaturing (6)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"