Overview

  • Product nameAnti-Robo1 antibody
    See all Robo1 primary antibodies
  • Description
    Rabbit polyclonal to Robo1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1600 to the C-terminus of Human Robo1.

    (Peptide available as ab94835.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).

Target

  • FunctionReceptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
  • Tissue specificityWidely expressed, with exception of kidney.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Ubiquitinated. May be deubiquitinated by USP33.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deleted in U twenty twenty antibody
    • DUTT 1 antibody
    • DUTT1 antibody
    • FLJ21882 antibody
    • H Robo 1 antibody
    • H-Robo-1 antibody
    • hRobo 1 antibody
    • Robo 1 antibody
    • Robo1 antibody
    • ROBO1_HUMAN antibody
    • Roundabout 1 antibody
    • Roundabout axon guidance receptor homolog 1 antibody
    • Roundabout homolog 1 antibody
    • Roundabout homolog1 precurser antibody
    • Roundabout1 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo1 antibody images

  • All lanes : Anti-Robo1 antibody (ab85312) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 180 kDa
    Observed band size : 190 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab85312 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85312, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Robo1 antibody (ab85312)

ab85312 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - Triton
Blocking step Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Jun 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Jun 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"