Overview

  • Product name
    Anti-Robo1 antibody - C-terminal
    See all Robo1 primary antibodies
  • Description
    Rabbit polyclonal to Robo1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Robo1 aa 1451-1636 (C terminal). The exact sequence is proprietary.
    Database link: Q9Y6N7

  • Positive control
    • HCC1937 LRobo1 (Lentivirus infection, carry Human Robo1); Human breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab184622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 181 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
  • Tissue specificity
    Widely expressed, with exception of kidney.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Ubiquitinated. May be deubiquitinated by USP33.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deleted in U twenty twenty antibody
    • DUTT 1 antibody
    • DUTT1 antibody
    • FLJ21882 antibody
    • H Robo 1 antibody
    • H-Robo-1 antibody
    • hRobo 1 antibody
    • Robo 1 antibody
    • Robo1 antibody
    • ROBO1_HUMAN antibody
    • Roundabout 1 antibody
    • Roundabout axon guidance receptor homolog 1 antibody
    • Roundabout homolog 1 antibody
    • Roundabout homolog1 precurser antibody
    • Roundabout1 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue, labeling Robo1 with ab184622 at 1/100 dilution.

  • All lanes : Anti-Robo1 antibody - C-terminal (ab184622) at 1/1000 dilution

    Lane 1 : HCC1937 Lx ( Lentivirus infection, carrying a contol vector)
    Lane 2 : HCC1937 LRobo1 (Lentivirus infection, carrying Human Robo1)

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 181 kDa

References

ab184622 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184622.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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