Overview

  • Product nameAnti-Robo2 antibody
    See all Robo2 primary antibodies
  • Description
    Mouse polyclonal to Robo2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length (Human) ROBO2, (NP_002933.1).

  • Positive control
    • ROBO2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 151 kDa. This antibody has only been tested against the transfected lysate.

Target

  • FunctionReceptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
  • Involvement in diseaseDefects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2) [MIM:610878]. VUR is a complex, genetically heterogeneous developmental disorder characterized by the retrograde flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause of 15% of end-stage renal disease in children and young adults.
    Note=A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominant-negative ROBO2 proteins that abrogate SLIT-ROBO signaling in vitro.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • lea antibody
    • Robo 2 antibody
    • Robo2 antibody
    • ROBO2_HUMAN antibody
    • Roundabout 2 antibody
    • Roundabout homolog 2 antibody
    • roundabout, axon guidance receptor, homolog 2 (Drosophila) antibody
    • Roundabout2 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo2 antibody images

  • All lanes : Anti-Robo2 antibody (ab72972) at 1/500 dilution

    Lane 1 : ROBO2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 151 kDa
    Observed band size : 151 kDa

References for Anti-Robo2 antibody (ab72972)

ab72972 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Oct 09 2012

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Oct 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"