Overview

  • Product nameAnti-Robo2 antibody
    See all Robo2 primary antibodies
  • Description
    Rabbit polyclonal to Robo2
  • Specificityab85278 does not cross react with Robo1 or Robo3.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding a region from the N-terminal amino acids 252-301 (

    PQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGK

    ) of human Robo2 (NP_002933)

  • Positive control
    • Human Fetal Brain Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 151 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionReceptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
  • Involvement in diseaseDefects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2) [MIM:610878]. VUR is a complex, genetically heterogeneous developmental disorder characterized by the retrograde flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause of 15% of end-stage renal disease in children and young adults.
    Note=A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominant-negative ROBO2 proteins that abrogate SLIT-ROBO signaling in vitro.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • lea antibody
    • Robo 2 antibody
    • Robo2 antibody
    • ROBO2_HUMAN antibody
    • Roundabout 2 antibody
    • Roundabout homolog 2 antibody
    • roundabout, axon guidance receptor, homolog 2 (Drosophila) antibody
    • Roundabout2 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo2 antibody images

  • Anti-Robo2 antibody (ab85278) at 1 µg/ml + Human Fetal Brain Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1: 50,000

    Predicted band size : 151 kDa
    Gel concentration: 6-18%
  • Lanes 1 - 4 : Anti-Robo2 antibody (ab85278) at 1/500 dilution
    Lanes 5 - 8 : Rabbit Polyclonal to V5 Tag

    Lane 1 : Empty
    Lane 2 : HEK293 cells expressing Robo1
    Lane 3 : HEK293 cells expressing Robo2-V5
    Lane 4 : HEK293 cells expressing Robo3
    Lane 5 : Empty
    Lane 6 : HEK293 cells expressing Robo1
    Lane 7 : HEK293 cells expressing Robo2-V5
    Lane 8 : HEK293 cells expressing Robo3

    Lysates/proteins at 20 µg per lane.

    Secondary
    Donkey Polyclonal to rabbit IgG Conjugated to HRP at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 151 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an abreview submitted by Alain Chedotal, Institut Vision, France

    See Abreview

References for Anti-Robo2 antibody (ab85278)

ab85278 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Oct 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Blocking step Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Oct 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"