Anti-Robo3 antibody (ab77261)


  • Product nameAnti-Robo3 antibody
    See all Robo3 primary antibodies
  • Description
    Goat polyclonal to Robo3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Dog, Zebrafish, Rhesus monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 915-926 of Human Robo3 (NP_071765.2).

  • Positive control
    • Human Brain (Cerebellum and Hippocampus) lysates.



Our Abpromise guarantee covers the use of ab77261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing.
    • Involvement in diseaseDefects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS) [MIM:607313]. Patients show a medulla where motor and sensory projections appear uncrossed.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
      Contains 3 fibronectin type-III domains.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ21044 antibody
      • HGPPS antibody
      • HGPS antibody
      • RB inhibiting gene 1 antibody
      • Rbig 1 antibody
      • Rbig1 antibody
      • Retinoblastoma inhibiting gene 1 antibody
      • Rig 1 antibody
      • Rig1 antibody
      • Robo 3 antibody
      • Robo3 antibody
      • Robo3 protein antibody
      • ROBO3_HUMAN antibody
      • Roundabout axon guidance receptor homolog 3 antibody
      • Roundabout homolog 3 antibody
      • Roundabout like protein 3 antibody
      • Roundabout, axon guidance receptor, homolog 3 (Drosophila) antibody
      • Roundabout-like protein 3 antibody
      see all

    Anti-Robo3 antibody images

    • Anti-Robo3 antibody (ab77261) at 0.5 µg/ml + human brain cerebellum lysate in RIPA buffer at 35 µg

      Predicted band size : 148 kDa
      Observed band size : 148 kDa

    References for Anti-Robo3 antibody (ab77261)

    ab77261 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Jan 29 2013


    Ablaufdatum: 05.März 2013
    Wert: €370.00

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
    Specification Brain, spinal cord
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

    Dr. Ruxandra Sirbulescu

    Verified customer

    Submitted Nov 14 2011