• Product nameAnti-Robo3 antibody
    See all Robo3 primary antibodies
  • Description
    Rabbit polyclonal to Robo3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Mouse Robo3.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: E14 Mouse Embryonic Spinal Cord; E11 Mouse Embryonic Brain.



Our Abpromise guarantee covers the use of ab99254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa).


  • FunctionThought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing.
  • Involvement in diseaseDefects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS) [MIM:607313]. Patients show a medulla where motor and sensory projections appear uncrossed.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21044 antibody
    • HGPPS antibody
    • HGPS antibody
    • RB inhibiting gene 1 antibody
    • Rbig 1 antibody
    • Rbig1 antibody
    • Retinoblastoma inhibiting gene 1 antibody
    • Rig 1 antibody
    • Rig1 antibody
    • Robo 3 antibody
    • Robo3 antibody
    • Robo3 protein antibody
    • ROBO3_HUMAN antibody
    • Roundabout axon guidance receptor homolog 3 antibody
    • Roundabout homolog 3 antibody
    • Roundabout like protein 3 antibody
    • Roundabout, axon guidance receptor, homolog 3 (Drosophila) antibody
    • Roundabout-like protein 3 antibody
    see all

Anti-Robo3 antibody images

  • All lanes : Anti-Robo3 antibody (ab99254) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Spinal Cord Tissue Lysate
    Lane 2 : E11 Mouse Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 146 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Robo3 antibody (ab99254)

ab99254 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C

Dr. Alain Chedotal

Verified customer

Submitted Jun 30 2012

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Alain Chedotal

Verified customer

Submitted Jun 23 2012