Overview

  • Product nameAnti-Robo4 antibody
    See all Robo4 primary antibodies
  • Description
    Rabbit polyclonal to Robo4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 245-275 of Human Robo4 (Q8WZ75)

  • Positive control
    • 293 cell line lysate, prostate carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab103674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 107 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • ECSM4 antibody
    • FLJ20798 antibody
    • Magic roundabout antibody
    • MGC133352 antibody
    • MGC133353 antibody
    • MRB antibody
    • Robo 4 antibody
    • ROBO4 antibody
    • ROBO4_HUMAN antibody
    • Roundabout homolog 4 antibody
    • Roundabout homolog 4 magic roundabout antibody
    • Roundabout homolog 4 Precursor antibody
    • Roundabout, axon guidance receptor, homolog 4 (Drosophila) antibody
    see all

Anti-Robo4 antibody images

  • Anti-Robo4 antibody (ab103674) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 107 kDa
  • IHC analysis of Robo4 expression in formalin fixed and paraffin embedded prostate carcinoma using 1/50 ab103674 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-Robo4 antibody (ab103674)

ab103674 has not yet been referenced specifically in any publications.

Product Wall

According to our records, ab39256, ab63319, ab77258, ab86673, ab103674 was proving difficult to use in [APPL] and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more detail...

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Thank you for your email.

We will look forward to receive the images. On the optimization note we strongly recommend to include the blocking step in protocol; please try 5%BSA or 5-10% normal serum for 1-2 hours at room temperature with specie...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"