Overview

  • Product nameAnti-ROC1 antibody
    See all ROC1 primary antibodies
  • Description
    Rabbit polyclonal to ROC1
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Synthetic peptide:

    CPLDNREWEFQKYGH

    conjugated to KLH, corresponding to C terminal amino acids 94-108 of Human ROC1 (NP_055063.1).

  • Positive control
    • Human brain (cerebellum) and placenta tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab116253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 12 kDa.
IP Use at an assay dependent concentration.
ELISA 1/2000 - 1/10000.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionE3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
  • Tissue specificityWidely expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BA554C12.1 antibody
    • E3 ubiquitin-protein ligase RBX1 antibody
    • FLJ60363 antibody
    • MGC13357 antibody
    • MGC1481 antibody
    • OTTHUMP00000028983 antibody
    • Protein ZYP antibody
    • Rbx 1 antibody
    • Rbx1 antibody
    • RBX1_HUMAN antibody
    • Regulator of cullins 1 antibody
    • Ring box 1 antibody
    • Ring box 1 E3 ubiquitin protein ligase antibody
    • RING box protein 1 antibody
    • RING finger protein 75 antibody
    • RING finger protein antibody
    • RING-box protein 1 antibody
    • Ringbox protein 1 antibody
    • RNF 75 antibody
    • RNF75 antibody
    • ROC 1 antibody
    • ZYP protein antibody
    see all

Anti-ROC1 antibody images

  • ab116253, at a 1/500 dilution, staining ROC1 in Formalin-fixed, Paraffin-embedded Human brain (cerebellum) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab116253, at a 1/500 dilution, staining ROC1 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-ROC1 antibody (ab116253)

ab116253 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"