• Product nameAnti-ROC1 antibody [EPR6849(B)]
    See all ROC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6849(B)] to ROC1
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human ROC1.

  • Positive control
    • HepG2, Jurkat and HeLa cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionE3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
    • Tissue specificityWidely expressed.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the RING-box family.
      Contains 1 RING-type zinc finger.
    • DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BA554C12.1 antibody
      • E3 ubiquitin-protein ligase RBX1 antibody
      • FLJ60363 antibody
      • MGC13357 antibody
      • MGC1481 antibody
      • OTTHUMP00000028983 antibody
      • Protein ZYP antibody
      • Rbx 1 antibody
      • Rbx1 antibody
      • RBX1_HUMAN antibody
      • Regulator of cullins 1 antibody
      • Ring box 1 antibody
      • Ring box 1 E3 ubiquitin protein ligase antibody
      • RING box protein 1 antibody
      • RING finger protein 75 antibody
      • RING finger protein antibody
      • RING-box protein 1 antibody
      • Ringbox protein 1 antibody
      • RNF 75 antibody
      • RNF75 antibody
      • ROC 1 antibody
      • ZYP protein antibody
      see all

    Anti-ROC1 antibody [EPR6849(B)] images

    • All lanes : Anti-ROC1 antibody [EPR6849(B)] (ab124950) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 12 kDa
      Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ROC1 antibody [EPR6849(B)] (ab124950)

    ab124950 has not yet been referenced specifically in any publications.

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