Anti-ROCK1 Cleavage Site (1113/1114) antibody [154C1465] (ab13660)

Overview

  • Product nameAnti-ROCK1 Cleavage Site (1113/1114) antibody [154C1465]
  • Description
    Mouse monoclonal [154C1465] to ROCK1 Cleavage Site (1113/1114)
  • SpecificityThis antibody recognizes the cleaved C-terminus (30kDa inhibitory domain) of ROCK1 only, after cleavage by Caspase 3. It does not recognize the full-length protein, and does not recognise the 130kDa active region. Since ROCK1 is cleaved by Caspase 3 during apoptosis, this antibody may be used for detection of Caspase 3 activation.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog, Human, Chimpanzee
  • Immunogen

    Synthetic peptide:

    GNLPESRIEGWLS

    , corresponding to C terminal amino acids 1114-1126 of Human ROCK1.

  • Positive control
    • Jurkat cells treated with camptothecin or with anti-Fas antibody.
  • General notes


    The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK1) has been found to be a new Caspase 3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK1 is cleaved by Caspase 3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The Caspase 3-mediated cleavage and activation of ROCK1 induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThe Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK1) has been found to be a new Caspase 3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK1 is cleaved by Caspase 3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The Caspase 3-mediated cleavage and activation of ROCK1 induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
  • ClonalityMonoclonal
  • Clone number154C1465
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 160 kDa).

Target

  • RelevanceROCK1 is a protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It is necessary for apoptotic membrane blebbing, and plays a role in smooth muscle contraction. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • p160 ROCK antibody
    • p160 ROCK1 antibody
    • Renal carcinoma antigen NY REN 35 antibody
    • Rho associated coiled coil containing protein kinase 1 antibody
    • Rho associated protein kinase 1 antibody
    • ROCK1 antibody
    see all

Anti-ROCK1 Cleavage Site (1113/1114) antibody [154C1465] images



  • Predicted band size : 160 kDa


    Detection of cleaved ROCK1 in apoptotic Jurkat cell lysates (10 µg/lane). Lane 1. Jurkat cells were treated with camptothecin. Lane 2. Jurkat cells were treated with anti-Fas antibody (10 ng/ml for 2 hrs). ab13660 was used at a concentration of 1 ug/ml.

References for Anti-ROCK1 Cleavage Site (1113/1114) antibody [154C1465] (ab13660)

ab13660 has not yet been referenced specifically in any publications.

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All the antibodies are well characterized, please choose based on your requirements. e.g polyclonal/ monoclonal, Rabbit/mouse/sheep etc.

The catalogue numbers are:

ab13660, ab45171, ab56661, a...

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Thank you for contacting us.

We unfortunately do not have anti ROCK antibodies available that can be used for functional assays e.g. activation or inhibition of ROCK. I would suggest searching the similar antibody clone in publications. We h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"