Anti-ROCK2 antibody (ab85612)


  • Product nameAnti-ROCK2 antibody
    See all ROCK2 primary antibodies
  • Description
    Rabbit polyclonal to ROCK2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N terminal of human ROCK2

  • Positive control
    • Human intestical cancer tissue; MCF7, HeLa, smmc, HT1080 and MM453 whole cell lysates.


Associated products


Our Abpromise guarantee covers the use of ab85612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa).Can be blocked with ROCK2 peptide (ab111859).
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionRegulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • coiled-coil-containing protein kinase 2 antibody
    • KIAA0619 antibody
    • p164 ROCK 2 antibody
    • p164 ROCK-2 antibody
    • Rho associated coiled coil containing protein kinase 2 antibody
    • Rho associated protein kinase 2 antibody
    • Rho associated, coiled coil containing protein kinase II antibody
    • Rho kinase 2 antibody
    • Rho-associated antibody
    • Rho-associated protein kinase 2 antibody
    • ROCK 2 antibody
    • Rock II antibody
    • Rock2 antibody
    • ROCK2_HUMAN antibody
    • Rock2m antibody
    • ROK alpha antibody
    • ROKalpha antibody
    see all

Anti-ROCK2 antibody images

  • All lanes : Anti-ROCK2 antibody (ab85612) at 1 µg/ml

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : smmc whole cell lysate
    Lane 4 : HT1080 whole cell lysate
    Lane 5 : MM453 whole cell lysate

    Predicted band size : 161 kDa
    Observed band size : 161 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ab85612 at 2µg/ml staining ROCK2 in human intestinal cancer by Immunohistochemistry, Paraffin-embedded tissue.

References for Anti-ROCK2 antibody (ab85612)

ab85612 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Brain tissue lysate, cytosolic fraction)
Loading amount 10 µg
Specification Brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

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Verified customer

Submitted Mar 14 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

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