The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml. IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in regulation of differentiation. In vitro, binds RNA, preferentially to both poly(G) and poly(U). Overexpressed, blocks differentiation of K562 leukemia cells following treatment with phorbol esters or sodium butyrate without affecting proliferation.
Tissue specificityExpressed in several hematopoietic cell lines examined.