Overview

  • Product nameAnti-ROM1 antibody
    See all ROM1 primary antibodies
  • Description
    Mouse polyclonal to ROM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human ROM1 protein (NP_000318.1)

  • Positive control
    • Human kidney lysate; ROM1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.

Target

  • FunctionMay function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. It is essential for disk morphogenesis.
  • Tissue specificityRetina (photoreceptor). In rim region of ROS (rod outer segment) disks.
  • Involvement in diseaseDefects in ROM1 may cause retinitis pigmentosa (RP) [MIM:268000]; when associated with defects in PRPH2.
  • Sequence similaritiesBelongs to the PRPH2/ROM1 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Retinal outer segment membrane protein 1 antibody
    • Rod outer segment membrane protein 1 antibody
    • ROM antibody
    • ROM1 antibody
    • ROM1_HUMAN antibody
    • ROSP1 antibody
    • RP7 antibody
    • Tetraspanin 23 antibody
    • Tetraspanin-23 antibody
    • Tspan 23 antibody
    • Tspan-23 antibody
    • TSPAN23 antibody
    see all

Anti-ROM1 antibody images

  • Anti-ROM1 antibody (ab72973) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ROM1 antibody (ab72973) at 1/500 dilution

    Lane 1 : ROM1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-ROM1 antibody (ab72973)

ab72973 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step normal goat serum (NGS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Cow Cell (bovine rom1 ortholog expressin in AD293)
Specification bovine rom1 ortholog expressin in AD293
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Submitted Aug 12 2013

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