Overview

  • Product nameAnti-ROM1 antibody
    See all ROM1 primary antibodies
  • Description
    Rabbit polyclonal to ROM1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (CCNPHSPRPC LQNRLSDSYA HPLFDPRQPN QNLWAQGCHE VLLEHLQDLA) of human ROM1 (NP_000318)

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionMay function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. It is essential for disk morphogenesis.
  • Tissue specificityRetina (photoreceptor). In rim region of ROS (rod outer segment) disks.
  • Involvement in diseaseDefects in ROM1 may cause retinitis pigmentosa (RP) [MIM:268000]; when associated with defects in PRPH2.
  • Sequence similaritiesBelongs to the PRPH2/ROM1 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Retinal outer segment membrane protein 1 antibody
    • Rod outer segment membrane protein 1 antibody
    • ROM antibody
    • ROM1 antibody
    • ROM1_HUMAN antibody
    • ROSP1 antibody
    • RP7 antibody
    • Tetraspanin 23 antibody
    • Tetraspanin-23 antibody
    • Tspan 23 antibody
    • Tspan-23 antibody
    • TSPAN23 antibody
    see all

Anti-ROM1 antibody images

  • Anti-ROM1 antibody (ab80507) at 1/1 dilution + transfected 293T cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

References for Anti-ROM1 antibody (ab80507)

This product has been referenced in:
  • Kevany BM  et al. Structural and functional analysis of the native peripherin-ROM1 complex isolated from photoreceptor cells. J Biol Chem 288:36272-84 (2013). WB ; Cow . Read more (PubMed: 24196967) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"