Overview

  • Product nameAnti-RON antibody
    See all RON primary antibodies
  • Description
    Rabbit polyclonal to RON
  • Tested applicationsSuitable for: Dot blot, WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to an internal region of Human RON. This sequence is identical in Mouse and Rat.

  • Positive control
    • MSP-treated RON-transfected HEK293 cell lysate
  • General notesab125283 is stable for 12 months from date of receipt when stored at –20°C to –70°C. ab125283 can be stored at 4°C for three month without detectable loss of activity.

Properties

Applications

Our Abpromise guarantee covers the use of ab125283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/2000.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 185 kDa (predicted molecular weight: 152 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml.

Target

  • FunctionReceptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
  • Tissue specificityKeratinocytes and lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • Post-translational
    modifications
    Proteolytic processing yields the two subunits.
    Phosphorylated in response to ligand binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c met related tyrosine kinase antibody
    • CD136 antibody
    • CD136 antigen antibody
    • CDw136 antibody
    • Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
    • Macrophage stimulating 1 receptor antibody
    • Macrophage stimulating protein receptor alpha chain antibody
    • MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
    • Macrophage stimulating protein receptor beta chain antibody
    • Macrophage-Stimulating 1 Receptor (MST1R) antibody
    • Macrophage-stimulating protein receptor beta chain antibody
    • MSP receptor antibody
    • Mst1r antibody
    • MST1R variant RON30 antibody
    • MST1R variant RON62 antibody
    • p185 RON antibody
    • p185-Ron antibody
    • Protein-tyrosine kinase 8 antibody
    • PTK 8 antibody
    • ptk8 antibody
    • PTK8 protein tyrosine kinase 8 antibody
    • Recepteur d’origine nantais (RON) antibody
    • RON antibody
    • RON protein tyrosine kinase antibody
    • RON variant E2E3 antibody
    • RON_HUMAN antibody
    • Soluble RON variant 1 antibody
    • Soluble RON variant 2 antibody
    • Soluble RON variant 3 antibody
    • Soluble RON variant 4 antibody
    • Stem cell derived tyrosine kinase antibody
    see all

Anti-RON antibody images

  • All lanes : Anti-RON antibody (ab125283) at 1/500 dilution

    Lane 1 : MSP-treated RON-transfected HEK293 cell lysate
    Lane 2 : MSP-treated RON-transfected HEK293 cell lysate with immunizing peptide


    Predicted band size : 152 kDa
    Observed band size : 185 kDa (why is the actual band size different from the predicted?)
  • Dot Blot: 1 µg peptide was blotted onto NC membrane. 1: RON (pY1238/Y1239). 2: RON (pY1238). 3: RON (pY1239). 4: RON (Nonphospho). 5: Non-related Phosphopeptide. ab125283 was used at a dilution of 1/2000.

References for Anti-RON antibody (ab125283)

ab125283 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"