Anti-RON (phospho S1394) antibody (ab61007)

Overview

  • Product nameAnti-RON (phospho S1394) antibody
    See all RON primary antibodies
  • Description
    Rabbit polyclonal to RON (phospho S1394)
  • SpecificityDetects endogenous levels of RON only when phosphorylated at serine 1394.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human RON around the phosphorylation site of serine 1394 (P-L-SP-E-P)

  • Positive control
    • WB: extracts from HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).
ELISA 1/40000.

Target

  • FunctionReceptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
  • Tissue specificityKeratinocytes and lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • Post-translational
    modifications
    Proteolytic processing yields the two subunits.
    Phosphorylated in response to ligand binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c met related tyrosine kinase antibody
    • CD136 antibody
    • CD136 antigen antibody
    • CDw136 antibody
    • Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
    • Macrophage stimulating 1 receptor antibody
    • Macrophage stimulating protein receptor alpha chain antibody
    • MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
    • Macrophage stimulating protein receptor beta chain antibody
    • Macrophage-Stimulating 1 Receptor (MST1R) antibody
    • Macrophage-stimulating protein receptor beta chain antibody
    • MSP receptor antibody
    • Mst1r antibody
    • MST1R variant RON30 antibody
    • MST1R variant RON62 antibody
    • p185 RON antibody
    • p185-Ron antibody
    • Protein-tyrosine kinase 8 antibody
    • PTK 8 antibody
    • ptk8 antibody
    • PTK8 protein tyrosine kinase 8 antibody
    • Recepteur d��origine nantais (RON) antibody
    • RON antibody
    • RON protein tyrosine kinase antibody
    • RON variant E2E3 antibody
    • RON_HUMAN antibody
    • Soluble RON variant 1 antibody
    • Soluble RON variant 2 antibody
    • Soluble RON variant 3 antibody
    • Soluble RON variant 4 antibody
    • Stem cell derived tyrosine kinase antibody
    see all

Anti-RON (phospho S1394) antibody images

  • All lanes : Anti-RON (phospho S1394) antibody (ab61007) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins)
    Lane 2 : extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins) with immunizing phosphopeptide


    Predicted band size : 152 kDa
    Observed band size : 152 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RON (phospho S1394) antibody (ab61007)

ab61007 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"