Anti-RON (phospho Y1238 + Y1239) antibody (ab125264)

Overview

  • Product nameAnti-RON (phospho Y1238 + Y1239) antibody
    See all RON primary antibodies
  • Description
    Rabbit polyclonal to RON (phospho Y1238 + Y1239)
  • Specificityab125264 recognizes RON when phosphorilated at Tyr 1238 and 1239.
  • Tested applicationsSuitable for: WB, IP, ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide containing the epitope -EPYYS-, with dual phosphorylation sites Y1238/9, from Human RON.

  • Positive control
    • Cell lysate from RON transfected HEK293. RON (pY1238/Y1239) peptide.

Properties

Applications

Our Abpromise guarantee covers the use of ab125264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 152 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
Dot blot 1/2000.

Target

  • FunctionReceptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
  • Tissue specificityKeratinocytes and lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • Post-translational
    modifications
    Proteolytic processing yields the two subunits.
    Phosphorylated in response to ligand binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c met related tyrosine kinase antibody
    • CD136 antibody
    • CD136 antigen antibody
    • CDw136 antibody
    • Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
    • Macrophage stimulating 1 receptor antibody
    • Macrophage stimulating protein receptor alpha chain antibody
    • MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
    • Macrophage stimulating protein receptor beta chain antibody
    • Macrophage-Stimulating 1 Receptor (MST1R) antibody
    • Macrophage-stimulating protein receptor beta chain antibody
    • MSP receptor antibody
    • Mst1r antibody
    • MST1R variant RON30 antibody
    • MST1R variant RON62 antibody
    • p185 RON antibody
    • p185-Ron antibody
    • Protein-tyrosine kinase 8 antibody
    • PTK 8 antibody
    • ptk8 antibody
    • PTK8 protein tyrosine kinase 8 antibody
    • Recepteur d’origine nantais (RON) antibody
    • RON antibody
    • RON protein tyrosine kinase antibody
    • RON variant E2E3 antibody
    • RON_HUMAN antibody
    • Soluble RON variant 1 antibody
    • Soluble RON variant 2 antibody
    • Soluble RON variant 3 antibody
    • Soluble RON variant 4 antibody
    • Stem cell derived tyrosine kinase antibody
    see all

Anti-RON (phospho Y1238 + Y1239) antibody images

  • All lanes : Anti-RON (phospho Y1238 + Y1239) antibody (ab125264) at 1/500 dilution

    Lane 1 : Cell lysate from MSP-treated RON transfected HEK293
    Lane 2 : Cell lysate from MSP-treated RON transfected HEK293 with immunizing peptide


    Predicted band size : 152 kDa
  • ab125264 at 1/2000 dilution detecting RON peptides by dot-blot analysis.
    1 µg of the following peptides were blotted onto NC membrane;

    A: RON (pY1238/Y1239)
    B: RON(pY1238)
    C: RON (pY1239)
    D: RON (Nonphospho);
    E: Non-related Phosphopeptide.

    The detection antibodies at a 1:2000 dilution were:
    1: ab15264; Rabbit anti-RON (pY1238/Y1239),
    2: Rabbit anti-RON (pY1238),
    3: Rabbit anti-RON (pY1239),
    4: Rabbit anti-RON

References for Anti-RON (phospho Y1238 + Y1239) antibody (ab125264)

ab125264 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"