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Rabbit monoclonal [EPR5598] to RON (phospho Y1353)
Only detects RON phosphorylated at Tyrosine 1353.
, ICC/IFmore detailsUnsuitable for:
Flow Cyt or IHC-P
Synthetic peptide within Human RON (C terminal) (phospho Y1353). The exact sequence is proprietary.
- A431 cell lysates treated with pervanadate; A431 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, PBS
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50000 - 1/200000. Detects a band of approximately 180 kDa (predicted molecular weight: 152 kDa).
||1/10 - 1/100.
||1/500 - 1/1000.
Is unsuitable for Flow Cyt or IHC-P.
Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
Keratinocytes and lung.
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
Proteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding.
Information by UniProt
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671)
All lanes : Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671) at 1/50000 dilution
Lane 1 : A431 cell lysates, untreated
Lane 2 : A431 cell lysates, treated with pervanadate
Predicted band size: 152 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671)
ab124671, at 1/500 dilution, staining RON in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.
Other - Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"