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Synthetic peptide: GLTELHDDLS NYIDGHTPEG SKADSAVSSF YLDIQPSPDQ SGLDINGIKP, corresponding to amino acids 217-266 of Human ROR alpha(Peptide available as ab151609.)
Our Abpromise guarantee covers the use of ab60134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|ChIP||Use at an assay dependent concentration. PubMed: 23922987|
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 63 kDa).Can be blocked with ROR alpha peptide (ab151609). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ROR alpha with ab60134 at a concentration of 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule.