Overview

  • Product name
  • Description
    Rabbit polyclonal to ROR alpha
  • Tested applications
    Suitable for: ICC/IF, ChIP, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: GLTELHDDLS NYIDGHTPEG SKADSAVSSF YLDIQPSPDQ SGLDINGIKP, corresponding to amino acids 217-266 of Human ROR alpha(Peptide available as ab151609.)

  • Positive control
    • Jurkat cells. Kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab60134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 23922987
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 63 kDa).Can be blocked with ROR alpha peptide (ab151609). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.
  • Tissue specificity
    Widely expressed in a number of tissues.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
    Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
    Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • fhl2a antibody
    • NR1F1 antibody
    • Nuclear receptor ROR-alpha antibody
    • Nuclear receptor RZR-alpha antibody
    • Nuclear receptor subfamily 1 group F member 1 antibody
    • RAR-related orphan receptor A antibody
    • Retinoid-related orphan receptor-alpha antibody
    • Rora antibody
    • RORA_HUMAN antibody
    • RZR-ALPHA antibody
    • RZRA antibody
    see all

Anti-ROR alpha antibody images

  • All lanes : Anti-ROR alpha antibody (ab60134) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ICC/IF image of ab60134 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ROR alpha with ab60134 at a concentration of 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule.

References for Anti-ROR alpha antibody (ab60134)

This product has been referenced in:
  • Tuong ZK  et al. Transgenic Adipose-specific Expression of the Nuclear Receptor RORa Drives a Striking Shift in Fat Distribution and Impairs Glycemic Control. EBioMedicine 11:101-117 (2016). Read more (PubMed: 27568222) »
  • Wang RX  et al. Melatonin downregulates nuclear receptor RZR/ROR? expression causing growth-inhibitory and anti-angiogenesis activity in human gastric cancer cells in vitro and in vivo. Oncol Lett 12:897-903 (2016). IHC ; Mouse . Read more (PubMed: 27446366) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Human Tissue lysate - whole (Human lung)
Specification
Human lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Mar 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Sample
Mouse Tissue sections (Lung tissue)
Specification
Lung tissue
Permeabilization
Yes - Tween 20 0.05%
Fixative
Formaldehyde
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Verified customer

Submitted Mar 17 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Sample
Human Tissue sections (Lung tissue)
Specification
Lung tissue
Permeabilization
Yes - 0.05% tween 20
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 14 2015

Thank you for your inquiry.

We offer normal tissue brain slides from mouse for the use in IHC-P. I recommend to have a look at the following product and if it might be suitable for you.

http://www.abcam.com/Brain-mouse-normal-tissue-s...

Read More

Many thanks for contacting us and your interest in our products.

I am pleased to be able to tell you we are able to provide the blocking peptide for the anti-ROR alpha antibody (ab60134). This can be viewed under the catalogue number ab151609:...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse adult retina)
Specification
mouse adult retina
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton-x100
Blocking step
10% FBS, 1%BSA as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: NA% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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