Anti-ROR alpha antibody - Carboxyterminal end (ab71016)


  • Product nameAnti-ROR alpha antibody - Carboxyterminal end
    See all ROR alpha primary antibodies
  • Description
    Rabbit polyclonal to ROR alpha - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C terminal region of human ROR alpha.

  • Positive control
    • Jurkat cell line lysates.


Associated products


Our Abpromise guarantee covers the use of ab71016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
ELISA 1/1000.


  • FunctionOrphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.
  • Tissue specificityWidely expressed in a number of tissues.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
    Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
    Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • fhl2a antibody
    • NR1F1 antibody
    • Nuclear receptor ROR-alpha antibody
    • Nuclear receptor RZR-alpha antibody
    • Nuclear receptor subfamily 1 group F member 1 antibody
    • RAR-related orphan receptor A antibody
    • Retinoid-related orphan receptor-alpha antibody
    • Rora antibody
    • RORA_HUMAN antibody
    • RZR-ALPHA antibody
    • RZRA antibody
    see all

Anti-ROR alpha antibody - Carboxyterminal end images

  • Anti-ROR alpha antibody - Carboxyterminal end (ab71016) at 1/300 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ROR alpha antibody - Carboxyterminal end (ab71016)

ab71016 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Cerebellum)
Loading amount 15 µg
Specification Cerebellum
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 17 2012

Thank you for your prompt answer. I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful. The attachment you have sent to me has the sequence data for human, ...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. The immunogen used to raise this antibody was a synth...

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