Overview

  • Product nameAnti-ROR gamma antibody [162C2a]
    See all ROR gamma primary antibodies
  • Description
    Mouse monoclonal [162C2a] to ROR gamma
  • Tested applicationsSuitable for: Dot blot, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of Human ROR gamma

Properties

Applications

Our Abpromise guarantee covers the use of ab58670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
WB
  • Application notesDot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively (PubMed:19965867, PubMed:22789990). Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORC-mediated activation of the expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism, including PLIN2 and AVPR1A (PubMed:19965867). Negative regulator of adipocyte differentiation through the regulation of early phase genes expression, such as MMP3. Controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. In liver, has specific and redundant functions with RORA as positive or negative modulator of expression of genes encoding phase I and Phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as SULT1E1. Also plays also a role in the regulation of hepatocyte glucose metabolism through the regulation of G6PC and PCK1 (PubMed:19965867). Regulates the rhythmic expression of PROX1 and promotes its nuclear localization (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Plays an indispensable role in the induction of IFN-gamma dependent anti-mycobacterial systemic immunity (PubMed:26160376).
      Isoform 2: Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes and Peyer's patches. Required for the generation of LTi (lymphoid tissue inducer) cells. Regulates thymocyte survival through DNA-binding on ROREs of target gene promoter regions and recruitment of coactivaros via the AF-2. Also plays a key role, downstream of IL6 and TGFB and synergistically with RORA, for lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. May also play a role in the pre-TCR activation cascade leading to the maturation of alpha/beta T-cells and may participate in the regulation of DNA accessibility in the TCR-J(alpha) locus.
    • Tissue specificityIsoform 1 is widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2 is primarily expressed in immature thymocytes.
    • Involvement in diseaseImmunodeficiency 42
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • DomainThe AF-2 (activation function-2) motif is required for recruiting coregulators containing LXXLL motifs such as NCOA1 and NCOA2.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC129539 antibody
      • NR1F3 antibody
      • Nuclear receptor ROR gamma antibody
      • Nuclear receptor ROR-gamma antibody
      • Nuclear receptor RZR gamma antibody
      • Nuclear receptor RZR-gamma antibody
      • Nuclear receptor subfamily 1 group F member 3 antibody
      • RAR related orphan receptor C antibody
      • RAR related orphan receptor C, isoform a antibody
      • RAR related orphan receptor gamma antibody
      • RAR-related orphan receptor C antibody
      • Retinoic acid binding receptor gamma antibody
      • Retinoid related orphan receptor gamma antibody
      • Retinoid-related orphan receptor-gamma antibody
      • Rorc antibody
      • RORG antibody
      • RORG_HUMAN antibody
      • RZR GAMMA antibody
      • RZRG antibody
      • TOR antibody
      see all

    Anti-ROR gamma antibody [162C2a] images

    References for Anti-ROR gamma antibody [162C2a] (ab58670)

    ab58670 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"