Overview

  • Product nameAnti-ROR1 antibody
    See all ROR1 primary antibodies
  • Description
    Mouse monoclonal to ROR1
  • Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Recombinant fragment: NCIRIGIPMA DPINKNHKCY NSTGVDYRGT VSVTKSGRQC QPWNSQYPHT HTFTALRFPE LNGGHSYCRN PGNQKEAPWC FTLDENFKSD LCDIPACGK, corresponding to amino acids 294-393 of Human ROR1 with a 26 kDa proprietary tag.

  • Positive control
    • Hela nuclear extract; recombinant ROR1 fragment (the immunogen).

Properties

Applications

Our Abpromise guarantee covers the use of ab93041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 104 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTyrosine-protein kinase receptor whose role is not yet clear.
  • Tissue specificityExpressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
    Contains 1 FZ (frizzled) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 kringle domain.
    Contains 1 protein kinase domain.
  • Developmental stageExpressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ537F10.1 antibody
    • MGC99659 antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor related 1 antibody
    • Neurotrophic tyrosine kinase, receptor related 1 antibody
    • NTRKR1 antibody
    • OTTHUMP00000010573 antibody
    • OTTHUMP00000010574 antibody
    • OTTMUSP00000008344 antibody
    • Receptor tyrosine kinase like orphan receptor 1 antibody
    • receptor-related 1 antibody
    • RGD1559469 antibody
    • ROR 1 antibody
    • ROR1 antibody
    • ROR1_HUMAN antibody
    • RP11 24J23.1 antibody
    • Tyrosine kinase like orphan receptor 1 antibody
    • Tyrosine protein kinase transmembrane receptor ROR1 antibody
    • Tyrosine-protein kinase transmembrane receptor ROR1 antibody
    see all

Anti-ROR1 antibody images

  • Overlay histogram showing A549 cells stained with ab93041 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93041, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Anti-ROR1 antibody (ab93041) at 1/500 dilution + Hela nuclear extract at 50 µg

    Predicted band size : 104 kDa
  • Anti-ROR1 antibody (ab93041) at 1/500 dilution + recombinant ROR1 protein fragment (the immunogen) at 0.2 µg

    Predicted band size : 104 kDa

References for Anti-ROR1 antibody (ab93041)

ab93041 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"