Overview

  • Product name
    Anti-ROR2 antibody [Nt 2535-2835]
    See all ROR2 primary antibodies
  • Description
    Mouse monoclonal [Nt 2535-2835] to ROR2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Mouse ROR2. Ror2-MBP (maltose binding protein) fusion of the mouse Ror2 protein (bases 2535-2835).

  • Positive control
    • ICC/IF: U20S cells. IHC-P: FFPE human prostate tissue sections. IHC-P: FFPE Human Stomach
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab190145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 105 kDa.
IHC-P Use a concentration of 10 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation.
  • Involvement in disease
    Brachydactyly B1
    Robinow syndrome autosomal recessive
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
    Contains 1 FZ (frizzled) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 kringle domain.
    Contains 1 protein kinase domain.
  • Developmental stage
    Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BDB antibody
    • BDB1 antibody
    • Brachydactyly type B antibody
    • EC 2.7.10.1 antibody
    • MGC163394 antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase, receptor related 2 antibody
    • NTRKR2 antibody
    • Receptor tyrosine kinase-like orphan receptor 2 antibody
    • receptor-related 2 antibody
    • ROR2 antibody
    • ROR2_HUMAN antibody
    • Tyrosine protein kinase transmembrane receptor ROR2 antibody
    • Tyrosine-protein kinase transmembrane receptor Ror2 antibody
    see all

Images



  • Predicted band size : 105 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ROR2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: MCF7 whole cell lysate (20 µg)
    Lane 4: U20S whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab190145 observed at 125 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab190145 was shown to recognize ROR2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when ROR2 knockout samples were examined. Wild-type and ROR2 knockout samples were subjected to SDS-PAGE. Ab190145 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-ROR2 antibody [Nt 2535-2835] (ab190145) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • IHC image of ROR2 staining in Normal Human Stomach formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F/B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab190145, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab190145 stained U20S cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190145 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • IHC image of ROR2 staining in Human normal prostate formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab190145, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Roarty K  et al. Ror2 regulates branching, differentiation, and actin-cytoskeletal dynamics within the mammary epithelium. J Cell Biol 208:351-66 (2015). Read more (PubMed: 25624393) »

See 1 Publication for this product

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