Overview

  • Product nameAnti-ROS1 antibody [4-6G]
    See all ROS1 primary antibodies
  • Description
    Mouse monoclonal [4-6G] to ROS1
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Extracellular portion of ROS amino acid 1-285 fused to Fc, transiently expressed in 293 cells and purified using PtnA column chromatography.

  • Positive control
    • Cells transiently expressing human ROS1, mouse cells expressing human ROS1.

Properties

Applications

Our Abpromise guarantee covers the use of ab108492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 264 kDa.

Target

  • FunctionThis is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity.
  • Tissue specificityExpressed in brain. Expression is increased in primary gliomas.
  • Involvement in diseaseNote=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 9 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c ros 1 antibody
    • c ros antibody
    • c ros oncogene 1 receptor tyrosine kinase antibody
    • c Ros receptor tyrosine kinase antibody
    • c ros1 antibody
    • MCF 3 antibody
    • MCF3 antibody
    • Oncogene ROS antibody
    • OTTHUMP00000017814 antibody
    • OTTHUMP00000017815 antibody
    • OTTHUMP00000040389 antibody
    • Proto oncogene c ros 1 protein antibody
    • Proto oncogene c Ros antibody
    • Proto oncogene tyrosine protein kinase ROS antibody
    • Proto oncogene tyrosine protein kinase ROS precursor antibody
    • Proto-oncogene c-Ros-1 antibody
    • Proto-oncogene tyrosine-protein kinase ROS antibody
    • Receptor tyrosine kinase c ros oncogene 1 antibody
    • Ros 1 antibody
    • ROS 1C antibody
    • ROS antibody
    • ROS proto oncogene 1 , receptor tyrosine kinase antibody
    • ROS_HUMAN antibody
    • ROS1 antibody
    • ROS1C antibody
    • Transmembrane tyrosine specific protein kinase antibody
    • v ros avian UR2 sarcoma virus oncogene homolog 1 antibody
    • v ros UR2 sarcoma virus oncogene homolog 1 antibody
    see all

References for Anti-ROS1 antibody [4-6G] (ab108492)

This product has been referenced in:
  • Deng G  et al. Downregulation of ROS-FIG inhibits cell proliferation, colony-formation, cell cycle progression, migration and invasion, while inducing apoptosis in intrahepatic cholangiocarcinoma cells. Int J Mol Med 34:661-8 (2014). WB, IHC-P ; Human . Read more (PubMed: 24968753) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Methanol
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Methanol
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"