Recombinant
RabMAb

Anti-ROS1 antibody [EPMGHR2] (ab189925)

Overview

  • Product name
    Anti-ROS1 antibody [EPMGHR2]
    See all ROS1 primary antibodies
  • Description
    Rabbit monoclonal [EPMGHR2] to ROS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    within Human ROS1 aa 2050-2150. The exact sequence is proprietary.
    Database link: P08922

  • Positive control
    • Human lung carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250.

See IHC antigen retrieval protocols.

Target

  • Function
    This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity.
  • Tissue specificity
    Expressed in brain. Expression is increased in primary gliomas.
  • Involvement in disease
    Note=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 9 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c ros 1 antibody
    • c ros antibody
    • c ros oncogene 1 receptor tyrosine kinase antibody
    • c Ros receptor tyrosine kinase antibody
    • c ros1 antibody
    • MCF 3 antibody
    • MCF3 antibody
    • Oncogene ROS antibody
    • OTTHUMP00000017814 antibody
    • OTTHUMP00000017815 antibody
    • OTTHUMP00000040389 antibody
    • Proto oncogene c ros 1 protein antibody
    • Proto oncogene c Ros antibody
    • Proto oncogene tyrosine protein kinase ROS antibody
    • Proto oncogene tyrosine protein kinase ROS precursor antibody
    • Proto-oncogene c-Ros-1 antibody
    • Proto-oncogene tyrosine-protein kinase ROS antibody
    • Receptor tyrosine kinase c ros oncogene 1 antibody
    • Ros 1 antibody
    • ROS 1C antibody
    • ROS antibody
    • ROS proto oncogene 1 , receptor tyrosine kinase antibody
    • ROS_HUMAN antibody
    • ROS1 antibody
    • ROS1C antibody
    • Transmembrane tyrosine specific protein kinase antibody
    • v ros avian UR2 sarcoma virus oncogene homolog 1 antibody
    • v ros UR2 sarcoma virus oncogene homolog 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ROS1 with Purified  ab189925 at 1:300 dilution (5.23 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ROS1 with unpurified ab189925 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. (Inset: negative control).

References

This product has been referenced in:
  • Rossi G  et al. Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives. Lung Cancer (Auckl) 8:45-55 (2017). IHC . Read more (PubMed: 28740441) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Antigen Retrival solution EDTA buffer pH9
Permeabilization
No
Specification
Kidney
Blocking step
Peroxidase as blocking agent for 15 minute(s) · Concentration: 0.03% · Temperature: 37°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Jun 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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