Recombinant Anti-ROS1 antibody [EPMGHR2] (ab189925)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPMGHR2] to ROS1
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ROS1 antibody [EPMGHR2]
See all ROS1 primary antibodies -
Description
Rabbit monoclonal [EPMGHR2] to ROS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung carcinoma tissue. WB: U-118 MG whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPMGHR2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/100-1/250. See IHC antigen retrieval protocols. |
WB |
1/1000. Predicted molecular weight: 264 kDa.
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Notes |
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IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/100-1/250. See IHC antigen retrieval protocols. |
WB
1/1000. Predicted molecular weight: 264 kDa. |
Target
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Function
This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in brain. Expression is increased in primary gliomas. -
Involvement in disease
Note=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6098 Human
- Omim: 165020 Human
- SwissProt: P08922 Human
- Unigene: 1041 Human
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Alternative names
- c ros 1 antibody
- c ros antibody
- c ros oncogene 1 receptor tyrosine kinase antibody
see all
Images
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All lanes : Anti-ROS1 antibody [EPMGHR2] (ab189925) at 1/1000 dilution
Lane 1 : U-118 MG (Human brain glioblastoma) whole cell lysate
Lane 2 : Saos-2 (Human osteosarcoma epithelial) whole cell lysate
Lane 3 : SW1353 (Human chondrosarcoma fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 264 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBSTWe advise using U-118MG (FIG-ROS1 fusion) or HCC78 (SLC34A2-ROS1 fusion) as a positive cell line by WB. We have been unable to identify a positive control cell line for endogenous full-length ROS1.
The identity of the lower MW band at approximately 60kDa is unknown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ROS1 with Purified ab189925 at 1:300 dilution (5.23 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ROS1 with unpurified ab189925 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin. (Inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab189925 has been referenced in 2 publications.
- Xu N et al. Ivermectin induces apoptosis of esophageal squamous cell carcinoma via mitochondrial pathway. BMC Cancer 21:1307 (2021). PubMed: 34876051
- Rossi G et al. Detection of ROS1 rearrangement in non-small cell lung cancer: current and future perspectives. Lung Cancer (Auckl) 8:45-55 (2017). IHC . PubMed: 28740441